scott@vtx-cpd.com

Replying to Sophie I. 11/11/2021 - 22:25

Sophie!

You are never too late for this CPD party!!!! How lovely to hear from you and thank you for supporting our course. Hope you enjoy and let me know if you have any questions at anytime.

Scott 🙂

Replying to Lucie T. 14/11/2021 - 14:50

Hello Lucie.

I hope you are well.

I am so glad you are enjoying the course, we really appreciate the support.

I will pass this on to Laura, were there ant particular videos/procedures you were looking for?

Scott 🙂

Replying to Megan B. 11/11/2021 - 22:46

Hello Megan.

I totally agree with Liz. If you can get a hold of a mucus trap and use a suction unit, it really does help with this technique:

https://burtonsveterinary.com/mucus-collection-trap-pack-of-10.html

Bronchoalveolar lavage (BAL) can be performed without endoscopic guidance when diffuse disease is present. This sampling method differs from those described above by providing sample from the lower airways. This technique is most successful in small to medium-sized pets. Sterile aspiration catheters are probably the best thing to use, but ultimately any soft catheter that is long enough could be considered.

The patient is anesthetised, intubated with a sterile endotracheal tube, and placed in lateral recumbency. If the disease process is more marked on one side, the patient should be positioned with that side down. An open-ended sterile aspiration catheter is passed through the endotracheal tube until it is gently wedged and cannot be advanced further. Withdrawing the catheter a few millimeters, rotating the catheter slightly and gently advancing again until wedged will help ensure that the catheter is wedged within an airway and not becoming lodged at an airway division. Once the catheter is in place, warmed sterile 0.9% saline is instilled through the catheter and immediately aspirated. The volume infused has not been standardized and recommendations vary from 5-30 mL aliquots to using 2-5 mL/kg. An additional aliquot may need to be infused to recover adequate volume. The volume of sample recovered should be 40-50% of the total volume instilled. After the sampling is complete, the patient is placed on 100% oxygen for about 5-10 minutes.

I hope that helps a little.

Scott 🙂

Replying to Anna Bassett 08/11/2021 - 21:21

Thank you so much Anna.

Your feedback is really helpful and really kind.

I am so pleased you are enjoying the course.

Scott 🙂

Replying to Emma F. 02/11/2021 - 20:37

Hey Emma.

Thank you so much for this. I will pass on to Jon. I am so pleased you are enjoying the course.

Scott 🙂

Replying to Emma Holt 21/08/2021 - 10:04

Emma,

Thanks again for your amazing answer here.

I just wanted to shar the full report from the pathologist:

Site Liver

Microscopic Description

Liver: Preservation is moderate and nucleated cellularity is low to moderate. Slide no4 contains
predominantly dense blood. The remaining scans contain variable amounts of fresh blood and small lipid
spaces. There are low to moderate numbers of well-differentiated hepatocytes arranged in sheets and clusters. Low numbers of erythrocytes and focal clusters contain low numbers of small clear punctate
vacuoles. There is rare focal moderate intracanalicullar bile stasis (bile casts). There are rare isolated
slender fusiform mesenchymal cells (presumed to be fibroblasts). Streaked nuclear material is frequently
associated with a parasite clusters and, in these areas, neutrophils and small lymphocytes occasionally
appear overrepresented. Infectious agents and atypical cells are not identified.

Microscopic Interpretation

Moderate focal cholestasis. Mild focal discrete vacuolar change.

Comments

Aspirates have harvested predominantly fresh blood however, within the hepatocytes there is evidence to
support cholestasis and although this is focal, it is moderate. The mild indiscrete vacuolar change is
non-specific and may be associated with elevated metabolic stress associated with inflammation of
varying aetiologies (hepatic and nonhepatic), as well as metabolic disease (e.g. pancreatitis).
Overt inflammation and infectious agents are not identified however this is a relatively small sample. The
fibroblast presence may be compatible with fibrosis however, this requires histopathology for definitive
diagnosis. Given the slightly increased numbers of leukocytes associated with hepatocytes, although overall
leukocyte numbers do not appear elevated mild inflammation cannot be excluded. Biopsy with
histopathology for evaluation of tissue architecture and tissue culture may be of value should changes
persist.

You basically were spot on!

Scott 🙂